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image pro plus software  (Bio-Rad)


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    Structured Review

    Bio-Rad image pro plus software
    Image Pro Plus Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 36153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+pro+plus+software/Image+Lab+Software/pmc13138048-61-6-9
    Average 99 stars, based on 36153 article reviews
    image pro plus software - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Software:

    Article Title: Molecular mechanism of interleukin-17A regulating airway epithelial cell ferroptosis based on allergic asthma airway inflammation.
    Article Snippet: Membranes were later rinsed three times using TBST, followed by incubation using goat anti-rabbit IgG (H + L) (Cat #: S0001, 1:5000, Affinity Biosciences, China) or goat anti-mouse IgG (H + L) HRP (Cat #: S0002, 1:5000, Affinity Biosciences, China) for 2 h at RT. .. Thereafter, a chemiluminescence reagent (Cat #: 1705061, Bio-Rad, USA) was utilized for protein band visualization, whereas quantitative signal analysis was carried out using Image-Pro Plus software. ..

    Article Title: Taxus chinensis var. mairei (Lemée et Lévl) Cheng et L.K. Fu overcomes the resistance to osimertinib in EGFR-mutant non-small-cell lung cancer via suppression of ERK1/2-related cholesterol biosynthesis.
    Article Snippet: .. Image Pro Plus software from Bio-Rad Laboratories was employed for the quantification of LDs in tumor tissue sections. ..

    Article Title: Molecular mechanism of interleukin-17A regulating airway epithelial cell ferroptosis based on allergic asthma airway inflammation
    Article Snippet: Membranes were later rinsed three times using TBST, followed by incubation using goat anti-rabbit IgG (H + L) (Cat #: S0001, 1:5000, Affinity Biosciences, China) or goat anti-mouse IgG (H + L) HRP (Cat #: S0002, 1:5000, Affinity Biosciences, China) for 2 h at RT. .. Thereafter, a chemiluminescence reagent (Cat #: 1705061, Bio-Rad, USA) was utilized for protein band visualization, whereas quantitative signal analysis was carried out using Image-Pro Plus software. ..

    Article Title: Hyperuricemia drives intestinal barrier dysfunction by regulating gut microbiota
    Article Snippet: After smashing using high throughput tissue grinder, supernatant containing total proteins was collected and resolved through by SDS-PAGE and transferred onto the polyvinylidene fluoride membranes (Millipore, MA, United States). .. The membranes were blocked with 5 % skimmed milk for 2 h at room temperature and then incubated with the following primary antibodies, mouse anti-TLR4 (Cat # sc-293072, 1:500, Santa Cruz Biotechnology, United States), rabbit anti-IL-1b (Cat # ab9722, 1:1000, Abcam), mouse anti-occludin (Cat # 33–1500, 1:1500, Thermo Fisher Scientific, United States) and rabbit anti-claudin-1 (Cat # NBP1-77036, 1:1000, Novus), rabbit anti-ZO-1 (Cat # AF5154, 1:1000, Affinity Biosciences, United States), rabbit anti–NF–κB p65(Cat # cst 8242, 1:1000, Cell Signaling Technology, United States) and rabbit anti-Phospho–NF–κB p65(Cat # cst 3033, 1:1000, Cell Signaling Technology, United States) at 4_C overnight, and then with the secondary antibodies at room temperature (RT) for 1 h. The protein signals were detected on an imaging system using a chemiluminescence kit (Millipore, MA, United States) and analyzed using Image-Pro Plus software (Bio-Rad, United States). ß-actin and GAPDH was used as the internal loading control. ..

    Article Title: A negative feedback loop between TET2 and leptin in adipocyte regulates body weight
    Article Snippet: To induce adipocyte differentiation, confluent 3T3-L1 cells were incubated with an adipogenic cocktail, including 0.5 mM isobutylmethylxanthine (IBMX, Sigma-Aldrich, Cat#15879-1 G), 1 μM dexamethasone (Sigma-Aldrich, Cat#D4902) and 10 μg/mL insulin (Gibco, Cat#12585014). .. Bio-Rad Image Lab and Image-Pro Plus software were used for analysis. .. Adipose tissues were rinsed in PBS, minced, and digested with 1 mg/mL type II collagenase (Worthington, Cat#LS004177) and 1% bovine serum albumin for 30 min at 37 °C with gentle agitation (120 rpm).

    Article Title: Hyperuricemia drives intestinal barrier dysfunction by regulating gut microbiota.
    Article Snippet: After smashing using high throughput tissue grinder, supernatant containing total proteins was collected and resolved through by SDS-PAGE and transferred onto the polyvinylidene fluoride membranes (Millipore, MA, United States). .. The membranes were blocked with 5 % skimmed milk for 2 h at room temperature and then incubated with the following primary antibodies, mouse anti-TLR4 (Cat# sc-293072, 1:500, Santa Cruz Biotechnology, United States), rabbit anti-IL-1b (Cat# ab9722, 1:1000, Abcam), mouse anti-occludin (Cat# 33–1500, 1:1500, Thermo Fisher Scientific, United States) and rabbit anti-claudin-1 (Cat# NBP1-77036, 1:1000, Novus), rabbit anti-ZO-1 (Cat# AF5154, 1:1000, Affinity Biosciences, United States), rabbit anti–NF–κB p65(Cat#cst 8242, 1:1000, Cell Signaling Technology, United States) and rabbit anti-Phospho–NF–κB p65(Cat# cst 3033, 1:1000, Cell Signaling Technology, United States) at 4_C overnight, and then with the secondary antibodies at room temperature (RT) for 1 h. The protein signals were detected on an imaging system using a chemiluminescence kit (Millipore, MA, United States) and analyzed using Image-Pro Plus software (Bio-Rad, United States). ß-actin and GAPDH was used as the internal loading control. ..

    Article Title: 1-NP hijacks endocrine-metabolic checkpoints and disrupts testicular steroidogenesis by suppressing the cAMP-PKA-CREB-HMGCR axis.
    Article Snippet: 1-Nitropyrene (1-NP), a representative reproductive toxicant enriched in nitro-PAHs, is a known reproductive toxicant.. Although our previous studies demonstrated that 1-NP impairs testosterone synthesis, its effects on other critical processes in testosterone biosynthesis, particularly cholesterol metabolism, remain unknown.. Using in vivo and in vitro models, we investigated 1-NP’s effects on cholesterol homeostasis and steroidogenesis.

    Article Title: Avian herpesvirus-specific LORF5 is a late gene, interacts with 19 viral and 111 host proteins, critical for virulence of Duck plague virus
    Article Snippet: .. Average plaque sizes were measured using Image-Pro Plus software (Bio-Rad, CA, USA). ..

    Incubation:

    Article Title: Hyperuricemia drives intestinal barrier dysfunction by regulating gut microbiota
    Article Snippet: After smashing using high throughput tissue grinder, supernatant containing total proteins was collected and resolved through by SDS-PAGE and transferred onto the polyvinylidene fluoride membranes (Millipore, MA, United States). .. The membranes were blocked with 5 % skimmed milk for 2 h at room temperature and then incubated with the following primary antibodies, mouse anti-TLR4 (Cat # sc-293072, 1:500, Santa Cruz Biotechnology, United States), rabbit anti-IL-1b (Cat # ab9722, 1:1000, Abcam), mouse anti-occludin (Cat # 33–1500, 1:1500, Thermo Fisher Scientific, United States) and rabbit anti-claudin-1 (Cat # NBP1-77036, 1:1000, Novus), rabbit anti-ZO-1 (Cat # AF5154, 1:1000, Affinity Biosciences, United States), rabbit anti–NF–κB p65(Cat # cst 8242, 1:1000, Cell Signaling Technology, United States) and rabbit anti-Phospho–NF–κB p65(Cat # cst 3033, 1:1000, Cell Signaling Technology, United States) at 4_C overnight, and then with the secondary antibodies at room temperature (RT) for 1 h. The protein signals were detected on an imaging system using a chemiluminescence kit (Millipore, MA, United States) and analyzed using Image-Pro Plus software (Bio-Rad, United States). ß-actin and GAPDH was used as the internal loading control. ..

    Article Title: Hyperuricemia drives intestinal barrier dysfunction by regulating gut microbiota.
    Article Snippet: After smashing using high throughput tissue grinder, supernatant containing total proteins was collected and resolved through by SDS-PAGE and transferred onto the polyvinylidene fluoride membranes (Millipore, MA, United States). .. The membranes were blocked with 5 % skimmed milk for 2 h at room temperature and then incubated with the following primary antibodies, mouse anti-TLR4 (Cat# sc-293072, 1:500, Santa Cruz Biotechnology, United States), rabbit anti-IL-1b (Cat# ab9722, 1:1000, Abcam), mouse anti-occludin (Cat# 33–1500, 1:1500, Thermo Fisher Scientific, United States) and rabbit anti-claudin-1 (Cat# NBP1-77036, 1:1000, Novus), rabbit anti-ZO-1 (Cat# AF5154, 1:1000, Affinity Biosciences, United States), rabbit anti–NF–κB p65(Cat#cst 8242, 1:1000, Cell Signaling Technology, United States) and rabbit anti-Phospho–NF–κB p65(Cat# cst 3033, 1:1000, Cell Signaling Technology, United States) at 4_C overnight, and then with the secondary antibodies at room temperature (RT) for 1 h. The protein signals were detected on an imaging system using a chemiluminescence kit (Millipore, MA, United States) and analyzed using Image-Pro Plus software (Bio-Rad, United States). ß-actin and GAPDH was used as the internal loading control. ..

    Imaging:

    Article Title: Hyperuricemia drives intestinal barrier dysfunction by regulating gut microbiota
    Article Snippet: After smashing using high throughput tissue grinder, supernatant containing total proteins was collected and resolved through by SDS-PAGE and transferred onto the polyvinylidene fluoride membranes (Millipore, MA, United States). .. The membranes were blocked with 5 % skimmed milk for 2 h at room temperature and then incubated with the following primary antibodies, mouse anti-TLR4 (Cat # sc-293072, 1:500, Santa Cruz Biotechnology, United States), rabbit anti-IL-1b (Cat # ab9722, 1:1000, Abcam), mouse anti-occludin (Cat # 33–1500, 1:1500, Thermo Fisher Scientific, United States) and rabbit anti-claudin-1 (Cat # NBP1-77036, 1:1000, Novus), rabbit anti-ZO-1 (Cat # AF5154, 1:1000, Affinity Biosciences, United States), rabbit anti–NF–κB p65(Cat # cst 8242, 1:1000, Cell Signaling Technology, United States) and rabbit anti-Phospho–NF–κB p65(Cat # cst 3033, 1:1000, Cell Signaling Technology, United States) at 4_C overnight, and then with the secondary antibodies at room temperature (RT) for 1 h. The protein signals were detected on an imaging system using a chemiluminescence kit (Millipore, MA, United States) and analyzed using Image-Pro Plus software (Bio-Rad, United States). ß-actin and GAPDH was used as the internal loading control. ..

    Article Title: Hyperuricemia drives intestinal barrier dysfunction by regulating gut microbiota.
    Article Snippet: After smashing using high throughput tissue grinder, supernatant containing total proteins was collected and resolved through by SDS-PAGE and transferred onto the polyvinylidene fluoride membranes (Millipore, MA, United States). .. The membranes were blocked with 5 % skimmed milk for 2 h at room temperature and then incubated with the following primary antibodies, mouse anti-TLR4 (Cat# sc-293072, 1:500, Santa Cruz Biotechnology, United States), rabbit anti-IL-1b (Cat# ab9722, 1:1000, Abcam), mouse anti-occludin (Cat# 33–1500, 1:1500, Thermo Fisher Scientific, United States) and rabbit anti-claudin-1 (Cat# NBP1-77036, 1:1000, Novus), rabbit anti-ZO-1 (Cat# AF5154, 1:1000, Affinity Biosciences, United States), rabbit anti–NF–κB p65(Cat#cst 8242, 1:1000, Cell Signaling Technology, United States) and rabbit anti-Phospho–NF–κB p65(Cat# cst 3033, 1:1000, Cell Signaling Technology, United States) at 4_C overnight, and then with the secondary antibodies at room temperature (RT) for 1 h. The protein signals were detected on an imaging system using a chemiluminescence kit (Millipore, MA, United States) and analyzed using Image-Pro Plus software (Bio-Rad, United States). ß-actin and GAPDH was used as the internal loading control. ..

    Article Title: 1-NP hijacks endocrine-metabolic checkpoints and disrupts testicular steroidogenesis by suppressing the cAMP-PKA-CREB-HMGCR axis.
    Article Snippet: 1-Nitropyrene (1-NP), a representative reproductive toxicant enriched in nitro-PAHs, is a known reproductive toxicant.. Although our previous studies demonstrated that 1-NP impairs testosterone synthesis, its effects on other critical processes in testosterone biosynthesis, particularly cholesterol metabolism, remain unknown.. Using in vivo and in vitro models, we investigated 1-NP’s effects on cholesterol homeostasis and steroidogenesis.

    Control:

    Article Title: Hyperuricemia drives intestinal barrier dysfunction by regulating gut microbiota
    Article Snippet: After smashing using high throughput tissue grinder, supernatant containing total proteins was collected and resolved through by SDS-PAGE and transferred onto the polyvinylidene fluoride membranes (Millipore, MA, United States). .. The membranes were blocked with 5 % skimmed milk for 2 h at room temperature and then incubated with the following primary antibodies, mouse anti-TLR4 (Cat # sc-293072, 1:500, Santa Cruz Biotechnology, United States), rabbit anti-IL-1b (Cat # ab9722, 1:1000, Abcam), mouse anti-occludin (Cat # 33–1500, 1:1500, Thermo Fisher Scientific, United States) and rabbit anti-claudin-1 (Cat # NBP1-77036, 1:1000, Novus), rabbit anti-ZO-1 (Cat # AF5154, 1:1000, Affinity Biosciences, United States), rabbit anti–NF–κB p65(Cat # cst 8242, 1:1000, Cell Signaling Technology, United States) and rabbit anti-Phospho–NF–κB p65(Cat # cst 3033, 1:1000, Cell Signaling Technology, United States) at 4_C overnight, and then with the secondary antibodies at room temperature (RT) for 1 h. The protein signals were detected on an imaging system using a chemiluminescence kit (Millipore, MA, United States) and analyzed using Image-Pro Plus software (Bio-Rad, United States). ß-actin and GAPDH was used as the internal loading control. ..

    Article Title: Hyperuricemia drives intestinal barrier dysfunction by regulating gut microbiota.
    Article Snippet: After smashing using high throughput tissue grinder, supernatant containing total proteins was collected and resolved through by SDS-PAGE and transferred onto the polyvinylidene fluoride membranes (Millipore, MA, United States). .. The membranes were blocked with 5 % skimmed milk for 2 h at room temperature and then incubated with the following primary antibodies, mouse anti-TLR4 (Cat# sc-293072, 1:500, Santa Cruz Biotechnology, United States), rabbit anti-IL-1b (Cat# ab9722, 1:1000, Abcam), mouse anti-occludin (Cat# 33–1500, 1:1500, Thermo Fisher Scientific, United States) and rabbit anti-claudin-1 (Cat# NBP1-77036, 1:1000, Novus), rabbit anti-ZO-1 (Cat# AF5154, 1:1000, Affinity Biosciences, United States), rabbit anti–NF–κB p65(Cat#cst 8242, 1:1000, Cell Signaling Technology, United States) and rabbit anti-Phospho–NF–κB p65(Cat# cst 3033, 1:1000, Cell Signaling Technology, United States) at 4_C overnight, and then with the secondary antibodies at room temperature (RT) for 1 h. The protein signals were detected on an imaging system using a chemiluminescence kit (Millipore, MA, United States) and analyzed using Image-Pro Plus software (Bio-Rad, United States). ß-actin and GAPDH was used as the internal loading control. ..



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